Detection of SARS-CoV-2 Genes in Stool Samples of COVID-19 Survivors Using the Real-Time Reverse Transcriptase Polymerase Chain Reaction Method

Authors

  • Ka’bah Diploma of Medical Laboratory Technology Study Program, Faculty of Health Technology, Universitas Megarezky, Makassar, Indonesia
  • Nur Laela Alydrus Diploma of Medical Laboratory Technology Study Program, Faculty of Health Technology, Universitas Megarezky, Makassar, Indonesia
  • Arlitha Dekayana Diploma of Medical Laboratory Technology Study Program, Faculty of Health Technology, Universitas Megarezky, Makassar, Indonesia
  • Kasmuddin Diploma of Medical Laboratory Technology Study Program, Faculty of Health Technology, Universitas Megarezky, Makassar, Indonesia
  • Rinaldi Indra Duma’ari Diploma of Medical Laboratory Technology Study Program, Faculty of Health Technology, Universitas Megarezky, Makassar, Indonesia

DOI:

https://doi.org/10.37275/amcr.v5i1.453

Keywords:

Feces, rRT-PCR, SARS-CoV-2

Abstract

The spread of a pneumonia outbreak infected with the new coronavirus (COVID-19) from Wuhan, China, has spread globally. Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has been identified as the pathogen responsible for the pneumonic plague. The angiotensin conversion enzyme (ACE2) is the cellular receptor for SARS-CoV-2 entry. ACE2 is highly expressed in the epithelium of the stomach, duodenum and rectum. In infected patients, some patients infected with SARS-CoV-2 experience unusual symptoms, namely gastrointestinal symptoms, namely diarrhea, nausea and vomiting. This study aims to detect the presence of the SARS-CoV-2 gene in stool samples from COVID-19 survivors using the method real time RT-PCR as the gold standard for confirming COVID-19 with the RdRp and Helicase (ORF1b) target genes. There were 12 samples of COVID-19 survivors who met the inclusion criteria. The results showed that of the 12 samples, 2 samples were positive for SARS-CoV-2 RNA with Ct values of 32.79 and 36.86 which were detected on days 18 and 22 days after their respiratory tract tests were negative.

Authors

  • Ka’bah1*
  • Nur Laela Alydrus1
  • Arlitha Dekayana1
  • Kasmuddin1
  • Rinaldi Indra Duma’ari1
  1. 1Diploma of Medical Laboratory Technology Study Program, Faculty of Health Technology, Universitas Megarezky, Makassar, Indonesia

Corresponding author Ka’bah — kabah.paharu@gmail.com

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Published

2024-01-02

Issue

Vol. 5 No. 1 (2024): Archives of The Medicine and Case Reports

Section

Articles

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How to Cite

Detection of SARS-CoV-2 Genes in Stool Samples of COVID-19 Survivors Using the Real-Time Reverse Transcriptase Polymerase Chain Reaction Method. (2024). Archives of The Medicine and Case Reports, 5(1), 557-561. https://doi.org/10.37275/amcr.v5i1.453

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